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Cat.No. : CSC-RR01246 Host Cell: HepG2
| Cat. No. | CSC-RR01246 |
| Description | This cell line is engineered to stably express human solute carrier family 10 member 1 (SLC10A1, also known as NTCP) in HepG2 cells. It is a useful cell model for studying human SLC10A1 in HepG2 cells. |
| Gene | SLC10A1 |
| Gene Species | Human |
| Host Cell | HepG2 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | This cell line is stable at least 10 passages. |
| Applications | Applied for researching human SLC10A1’s role in liver physiology/pathophysiology (e.g., bile acid homeostasis), screening compounds targeting SLC10A1, and analyzing SLC10A1-associated signaling in liver cancer models. |
| Size Form | One vial of frozen cells, typically >1x10^6cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent cell line |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
This stable HepG2 line has become a staple in our lab for bile acid transport studies. The overexpression of SLC10A1 is consistent, yielding reproducible data in our uptake assays. It is a robust cellular model for toxicology and drug transport research. We appreciate the technical support provided by the Creative Biogene team as well.
Canada
03/25/2025
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