Human SLC15A1 Stable Cell Line - HeLa

Name: Human SLC15A1 Stable Cell Line - HeLa
SKU: CSC-RO02558
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO02558

Host Cell : HeLa Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO02558
Description	This cell line is engineered to stably overexpress Human SLC15A1 in HeLa cell line. GFP reporter gene is also expressed in this cell line for cell tracking.
Product Type	Stable cell line constitutively expressing target gene
Target Gene	SLC15A1
Gene Species	Human
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	Gene function studies and others
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Detection of gene overexpression by Real-time qPCR ,or western blot, or flow cytometry, 2) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

SLC15A1

Background

This gene encodes an intestinal hydrogen peptide cotransporter that is a member of the solute carrier family 15. The encoded protein is localized to the brush border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into the enterocytes. This protein plays an important role in the uptake and digestion of dietary proteins. This protein also facilitates the absorption of numerous peptidomimetic drugs. [provided by RefSeq, Apr 2010]

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