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Human RIPK3 Stable Cell Line - Hep G2

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01325

Host Cell :   Huh7 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO01325
Description This cell line is engineered to stably express Homo sapiens (human) receptor interacting serine/threonine kinase 3 (RIPK3) in Human hepatocellular carcinoma / hepatoma / liver cancer cell line (HuH-7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type Human gene overexpression stable cell line
Target Gene RIPK3
Gene Species Homo sapiens (human)
Host Cell Huh7
Host Cell Species Homo sapiens (Human)
Reporter GFP
Applications 1) Investigation of gene function
2) Screening and validation of antibodies
Size >1x10^6 frozen cells/vial, 1 mL
Stability Stable in culture over a minimum of 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene RIPK3
Background The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]
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Work well

Since Hep G2 cells often have low endogenous RIPK3, this stable line was exactly what we needed to study necroptotic pathways in liver cells. The cells are highly sensitive to necroptosis induction, validating the functional integrity of the inserted RIPK3 gene.

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