Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01325
Host Cell : Huh7 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01325 |
| Description | This cell line is engineered to stably express Homo sapiens (human) receptor interacting serine/threonine kinase 3 (RIPK3) in Human hepatocellular carcinoma / hepatoma / liver cancer cell line (HuH-7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells. |
| Product Type | Human gene overexpression stable cell line |
| Target Gene | RIPK3 |
| Gene Species | Homo sapiens (human) |
| Host Cell | Huh7 |
| Host Cell Species | Homo sapiens (Human) |
| Reporter | GFP |
| Applications |
1) Investigation of gene function 2) Screening and validation of antibodies |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Stability | Stable in culture over a minimum of 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | RIPK3 |
| Background | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008] |
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Since Hep G2 cells often have low endogenous RIPK3, this stable line was exactly what we needed to study necroptotic pathways in liver cells. The cells are highly sensitive to necroptosis induction, validating the functional integrity of the inserted RIPK3 gene.
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