Human PLOD2 KO Cell Line-HEK293T

Name: Human PLOD2 KO Cell Line-HEK293T
SKU: CSC-RT2885
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2885

Target Gene : PLOD2 Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2885
Description	This cell is a stable cell line with a homozygous knockout of human PLOD2 using CRISPR/Cas9.
Target Gene	PLOD2
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PLOD2 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 [ Homo sapiens ]
Gene Symbol	PLOD2
Synonyms	LH2; TLH
Gene Description	procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Gene ID	5352
Uni Prot ID	O00469
m RNA Refseq	NM_000935.2
Protein Refseq	NP_000926.2
Chromosome Location	3q24
Function	L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; procollagen-lysine 5-dioxygenase activity;
Pathway	Collagen biosynthesis and modifying enzymes, organism-specific biosystem; Collagen formation, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem;
MIM	601865

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