Human MAPT Knockout Cell Line-HCT116

Name: Human MAPT Knockout Cell Line-HCT116
SKU: CSC-RT2825
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2825

Target Gene : MAPT Host Cell : HCT116

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2825
Description	This cell is a stable cell line with a homozygous knockout of human MAPT using CRISPR/Cas9.
Target Gene	MAPT
Host Cell	HCT116
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MAPT microtubule-associated protein tau [ Homo sapiens ]
Gene Symbol	MAPT
Synonyms	TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
Gene Description	microtubule-associated protein tau
GeneID	4137
Uni ProtID	P10636
mRNA Refseq	NM_001123066.3
Protein Refseq	NP_001116538.2
Chromosome Location	17q21.1
Function	SH3 domain binding; apolipoprotein binding; enzyme binding; lipoprotein particle binding; microtubule binding; protein binding; protein kinase binding; structural constituent of cytoskeleton;
Pathway	Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Alzheimers Disease, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic cleavage of cellular proteins, organism-specific biosystem; Apoptotic execution phase, organism-specific biosystem; Caspase-mediated cleavage of cytoskeletal proteins, organism-specific biosystem;
MIM	157140

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