Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR01253
Host Cell : HEK293 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR01253 |
| Description | HEK293-Human-KDR/NFAT-Luc cell line is engineered to stably express human KDR (VEGFR2) and a luciferase reporter system under the control of NFAT response element. The overexpression of human TGFBR2 has been validated by QPCR analysis, and the functional activity of this cell line has been verified through VEGF165 stimulation treatment. |
| Product Type | Signaling Pathway Reporter Cell Lines |
| Target Gene | KDR/NFAT-Luc |
| Gene Species | Human |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Used to detect KDR (VEGFR2)-mediated NFAT signaling (e.g., in response to VEGF); applicable for studying angiogenesis, vascular biology, or screening anti-angiogenic drugs targeting KDR. |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent cell line |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Creative Biogene’s KDR/NFAT reporter line has become a staple in our angiogenesis lab. The cells are stable over multiple passages and respond consistently to VEGF stimulation. It is a highly reliable tool for our high-throughput drug screening assays.
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