Human FGF2 KO Cell Line-Hela

Name: Human FGF2 KO Cell Line-Hela
SKU: CSC-RT2835
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2835

Target Gene : FGF2 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2835
Description	This cell is a stable cell line with a homozygous knockout of human FGF2 using CRISPR/Cas9.
Target Gene	FGF2
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens ]
Gene Symbol	FGF2
Synonyms	BFGF; FGFB; FGF-2; HBGF-2
Gene Description	fibroblast growth factor 2 (basic)
GeneID	2247
Uni ProtID	P09038
mRNA Refseq	NM_002006.4
Protein Refseq	NP_001997.5
Chromosome Location	4q26
Function	chemoattractant activity; cytokine activity; fibroblast growth factor binding; fibroblast growth factor receptor binding; growth factor activity; heparin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; voltage-gated calcium channel activity;
Pathway	Activated point mutants of FGFR2, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Angiogenesis, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Constitutive PI3K/AKT Signaling in Cancer, organism-specific biosystem; DAP12 interactions, organism-specific biosystem; DAP12 signaling, organism-specific biosystem;
MIM	134920

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