Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR01256
Host Cell : HEK293 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR01256 |
| Description | HEK293-Human-EGFR/STAT3-Luc cell line is engineered to stably express human EGFR and a luciferase reporter system under the control of STAT3 response element. The overexpression of human EGFR has been validated by QPCR analysis, and the functional activity of this cell line has been verified through EGF stimulation treatment. |
| Product Type | Signaling Pathway Reporter Cell Lines |
| Target Gene | EGFR/STAT3-Luc |
| Gene Species | Human |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Used to assess EGFR (epidermal growth factor receptor)-mediated STAT3 activation; applicable for studying EGFR signaling in cell growth/survival, screening EGFR inhibitors, or investigating EGFR-related cancer mechanisms. |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent cell line |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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This cell line provided exactly what we needed to study the STAT3 downstream pathway upon EGFR activation. The cells are robust, easy to culture, and show a distinct, quantifiable response to EGF.
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