Human BRD2 Knockout Cell Line-HEK293T

Name: Human BRD2 Knockout Cell Line-HEK293T
SKU: CSC-RT2798
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2798

Target Gene : BRD2 Host Cell : HEK293T

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2798
Description	This cell is a stable cell line with a homozygous knockout of human BRD2 using CRISPR/Cas9.
Target Gene	BRD2
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BRD2 bromodomain containing 2 [ Homo sapiens ]
Gene Symbol	BRD2
Synonyms	FSH; NAT; RNF3; FSRG1; RING3; D6S113E
Gene Description	bromodomain containing 2
Gene ID	6046
Uni Prot ID	P25440
m RNA Refseq	NM_005104.3
Protein Refseq	NP_005095.1
Chromosome Location	6p21.3
Function	chromatin binding; histone acetyl-lysine binding;
Pathway	Regulation of retinoblastoma protein, organism-specific biosystem;
MIM	601540

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