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Human BMPR1A/SMAD1584-Luc Reporter Cell Line - HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01258

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RR01258
Description HEK293-Human-BMPR1A/SMAD1584-Luc cell line is engineered to stably express human BMPR1A and a luciferase reporter system under the control of SMAD1/5/8/4 response element. The overexpression of human BMPR2 has been validated by QPCR analysis, and the functional activity of this cell line has been verified through BMP2 stimulation treatment.
Product Type Signaling Pathway Reporter Cell Lines
Target Gene BMPR1A/SMAD1584
Gene Species Human
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications Applied to monitor BMPR1A (bone morphogenetic protein receptor 1A)-driven SMAD1/5/8/4 signaling; suitable for studying BMP pathway functions, evaluating BMPR1A-targeted compounds, or investigating BMP-related disorders.
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Highly recommended!

We used this line to test the efficacy of various BMP ligands. The performance is consistent from batch to batch, and the cells maintain their phenotype well. Creative Biogene provided excellent technical support to get us started.

United Kingdom

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