Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01329
Host Cell : Huh7 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01329 |
| Description | This cell line is engineered to stably express Homo sapiens (human) asparagine synthetase (glutamine-hydrolyzing) (ASNS) in Human hepatocellular carcinoma / hepatoma / liver cancer cell line (HuH-7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells. |
| Product Type | Human gene overexpression stable cell line |
| Target Gene | ASNS |
| Gene Species | Homo sapiens (human) |
| Host Cell | Huh7 |
| Host Cell Species | Homo sapiens (Human) |
| Reporter | GFP |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | ASNS |
| Background | The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] |
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The Human ASNS Stable Cell Line - Hep G2 from Creative Biogene exceeded our expectations in studying amino acid metabolism. The expression level of ASNS was consistent across passages, and the cells maintained excellent viability.
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