Human ADAR Knockout Cell Line-HEK293

Name: Human ADAR Knockout Cell Line-HEK293
SKU: CSC-RT2803
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2803

Target Gene : ADAR Host Cell : HEK293

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2803
Description	This cell is a stable cell line with a homozygous knockout of human ADAR using CRISPR/Cas9.
Target Gene	ADAR
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ADAR adenosine deaminase, RNA-specific [ Homo sapiens ]
Gene Symbol	ADAR
Synonyms	DSH; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP
Gene ID	103
Uni Prot ID	P55265
m RNA Refseq	NM_001025107.2
Protein Refseq	NP_001020278.1
Chromosome Location	1q21.3
Function	DNA binding; double-stranded RNA adenosine deaminase activity; double-stranded RNA adenosine deaminase activity; double-stranded RNA binding; metal ion binding; protein binding;
Pathway	C6 deamination of adenosine, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Formation of editosomes by ADAR proteins, organism-specific biosystem; Gene Expression, organism-specific biosystem; Immune System, organism-specific biosystem;
MIM	146920

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