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CC-1636

CRISPR Cas13a RNA Detection Kit (Two-step, Freeze-dried, Isothermal-Lateral Flow)

For research use only. Not intended for any clinical use.

Cat. No. :   CC-1636

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Product Information

Cat. No. CC-1636
Description Target RNA is transcribed into RNA by RNA polymerase. Cas13a forms a complex with crRNA, pairs with target RNA, and is activated to trans-cleave ssRNA. Uses upgraded LwaCas13a protein with 50% higher activity than wild type for enhanced RNA detection.
Gene Abbr Cas13a
Features 50% increased activity, ultra-sensitive, rapid detection
Applications Molecular diagnostics, pathogen detection
Manipulation After isothermal amplification, perform Cas13a detection at 37°C for 30–60 min, dilute and apply to lateral flow strip
Sensitivity 10–100 copies/test
Reactions 96T
Kit Components Reaction Buffer (2X), Reaction Tube, Positive Control (10X), Cleavage Buffer (10X), Trans Mix (5X), Reporter (4 μM), T7 RNA Polymerase (40X), Cas13a Protein (10 μM), Cas13a Diluent Buffer, crRNA for Positive Control (20X), Starter (10X), Diluent
Storage Store at -20°C
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