CRISPR Cas13a RNA Detection Kit (Two-step, Freeze-dried, Isothermal-Fluorescence)

Name: CRISPR Cas13a RNA Detection Kit (Two-step, Freeze-dried, Isothermal-Fluorescence)
SKU: CC-1637

For research use only. Not intended for any clinical use.

Cat.No.

CC-1637

Description

Cas13a forms a complex with crRNA and slides along target RNA. Activated Cas13a trans-cleaves reporters. Combines isothermal amplification with Cas13a, offering high sensitivity, specificity, and signal-to-noise ratio.

Applications

Molecular diagnostics, rapid and precise pathogen detection

Features

High sensitivity, specificity, signal-to-noise ratio

Manipulation

After isothermal amplification, perform Cas13a detection at 37°C for 30–60 min, read FAM signal

Sensitivity

10–100 copies/test

Storage

Store at -20°C

Classification

CRISPR Detection Kit

GeneAbbr

Cas13a

Reactions

96T

Kit Components

Reaction Buffer (2X), Reaction Tube, Positive Control (10X), Cleavage Buffer (10X), Trans Mix (5X), T7 RNA Polymerase (40X), Reporter (4 μM), Cas13a Protein (10 μM), Cas13a Diluent Buffer, crRNA for Positive Control (20X), Starter (10X)

Publications

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