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Cas13a Reaction Kit (Fluorescence)

For research use only. Not intended for any clinical use.
Cat.No.
CC-1599
Description
DNA template with T7 promoter is transcribed into RNA by RNA polymerase. Cas13a forms a complex with crRNA, pairs with target RNA, and is activated to trans-cleave nearby ssRNA. Uses upgraded LwaCas13a protein with 50% higher activity, improving detection.
Applications
Molecular diagnostics, pathogen detection
Features
50% increased activity
Manipulation
Thaw on ice, mix reagents. Prepare 20 μL reaction, incubate at 37°C for 10-30 min, read FAM signal

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