Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CC-1625
| Cat. No. | CC-1625 |
| Description | DNA template with T7 promoter is transcribed into RNA by RNA polymerase. Cas13a forms a complex with crRNA, pairs with target RNA, and is activated to trans-cleave nearby ssRNA. Uses upgraded LwaCas13a protein with 50% higher activity, improving detection. |
| Gene Abbr | Cas13a |
| Features | 50% increased activity |
| Applications | Molecular diagnostics, pathogen detection |
| Manipulation | Thaw on ice, mix reagents. Prepare 20 μL reaction, incubate at 37°C for 10-30 min, read FAM signal |
| Reactions | 96T |
| Kit Components | Cleavage Buffer (10X), Trans Mix (5X), T7 RNA Polymerase (40X), Cas13a Protein (10 μM), Cas13a Diluent Buffer, Positive Control (10X), Reporter (4 μM), crRNA for Positive Control (20X) |
| Storage | Store at -20°C |
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