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CC-1625

Cas13a Reaction Kit (Fluorescence)

For research use only. Not intended for any clinical use.

Cat. No. :   CC-1625

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Product Information

Cat. No. CC-1625
Description DNA template with T7 promoter is transcribed into RNA by RNA polymerase. Cas13a forms a complex with crRNA, pairs with target RNA, and is activated to trans-cleave nearby ssRNA. Uses upgraded LwaCas13a protein with 50% higher activity, improving detection.
Gene Abbr Cas13a
Features 50% increased activity
Applications Molecular diagnostics, pathogen detection
Manipulation Thaw on ice, mix reagents. Prepare 20 μL reaction, incubate at 37°C for 10-30 min, read FAM signal
Reactions 96T
Kit Components Cleavage Buffer (10X), Trans Mix (5X), T7 RNA Polymerase (40X), Cas13a Protein (10 μM), Cas13a Diluent Buffer, Positive Control (10X), Reporter (4 μM), crRNA for Positive Control (20X)
Storage Store at -20°C
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