CALML3 Easy KO Kit

Name: CALML3 Easy KO Kit
SKU: CC-245
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-245

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Product Information

Gene Information

Cat. No.	CC-245
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	CALML3
Species	Human
Ensembl ID	ENSG00000178363
NCBIGene ID	810
Uni Prot ID	P27482
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Target Gene	CALML3
Background	Predicted to enable calcium ion binding activity. Located in extracellular exosome. [provided by Alliance of Genome Resources, Feb 2025]

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