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ABCC1 Easy KO Kit

For research use only. Not intended for any clinical use.
Cat.No.
CC-13
Description
A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Applications
This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Features
  • All-in-One workflow from gene editing to knockout validation for users with no prior experience.
  • Pre-validated sgRNAs and primers for rapid setup.
  • Streamlined experiment handling.
  • CRISPR RNP method ensures precise and efficient gene knockout.
Species
Human

Publications

Q & A

Customer Reviews

Customer Q&As
How is CRISPR-Cas9 used in rice?

A: Currently, CRISPR/Cas9 technology is widely used by researchers for genome editing to achieve the desired biological objectives, because of its simple targeting. Over the past few years many genes that are related to various aspects of rice grain quality have been successfully edited via CRISPR/Cas9 technology.

What is the genome size of rice?

A: The rice genome is well mapped and well characterized, and it is the smallest of the major cereal crop genomes at an estimated 400 to 430 Mb.

Why does rice have more genes than humans?

A: The researchers theorize that rice has more genes than humans do because in plants, protein diversity depends on gene duplication. In humans, protein diversity depends not only on gene duplication, but also, on alternative splicing.

Can gene editing improve rice yield?

A: Yes, turning off a particular gene in rice could enhance grain yields by 8 percent, according to a new study.

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Customer Reviews
Great Kit

Great kit. The Rice CRISPR/Cas9 Genome Editing Kit helped me successfully knock-out the endogenous MYB30, a cold tolerance gene, simultaneously with other two yield related genes.

United States

12/02/2021

Useful Tool

Great buy! Four genes, REC8, PAIR, OSD1, and MTL were edited using Rice CRISPRRice CRISPR/Cas9 Genome Editing Kit for heterozygosity fixation and haploid induction.

United States

02/06/2022

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