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pMV261

For research use only. Not intended for any clinical use.
Cat.No.
VET1295
Background
Plasmid DNA pMV261 is a replication vector (multi-copy, extrachromosomal) containing a DNA cassette encoding kanamycin resistance (Tn903-derived aph gene), an E. coli origin of replication (oriE), and a mycobacterial plasmid DNA origin of replication (oriM), an expression cassette containing a mycobacterial promoter, multiple cloning site, and transcription terminator.
Promoter
Mycobacterium bovis hsp60
Resistance
Kanamycin
Vector Size
4488 bp
Vector Type
Expression Vector

Applications

Publications

Q & A

Customer Reviews

The pMV261 vector is a widely used tool in molecular biology research. It is a plasmid vector that is commonly used for gene cloning and expression studies in bacterial systems. One of the main applications of the pMV261 vector is gene cloning. This vector provides a suitable backbone for the insertion of foreign DNA sequences. Researchers can insert their gene of interest into the multiple cloning site (MCS) of the pMV261 vector, which contains a set of unique restriction enzyme sites. Another important application of the pMV261 vector is gene expression studies. The vector contains a strong promoter sequence, which allows for efficient transcription of the inserted gene. Moreover, the pMV261 vector can be used for protein expression studies. The vector contains a sequence that facilitates the production of recombinant proteins in bacteria. Researchers can insert their gene of interest into the vector, where it will be transcribed and translated into a protein of interest. This protein can then be purified and analyzed for its structure and function. Overall, the pMV261 vector is a versatile tool in molecular biology research that allows for gene cloning, gene expression studies, and protein expression.
Customer Q&As
What is the promoter used in pMV261?

A: The promoter used in pMV261 is Hsp60.

Who is the source/author of pMV261?

A: pMV261 was described in the publication by Stover et al. in 1991, Nature 351:456.

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Customer Reviews
High copy number

pMV261 is a high-copy number plasmid, which means that it can replicate in large quantities within the host cell. This is advantageous for obtaining high expression levels of the recombinant protein.

United States

10/01/2021

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