Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1295
| Cat. No. | VET1295 |
| Background | Plasmid DNA pMV261 is a replication vector (multi-copy, extrachromosomal) containing a DNA cassette encoding kanamycin resistance (Tn903-derived aph gene), an E. coli origin of replication (oriE), and a mycobacterial plasmid DNA origin of replication (oriM), an expression cassette containing a mycobacterial promoter, multiple cloning site, and transcription terminator. |
| Promoter | Mycobacterium bovis hsp60 |
| Resistance | Kanamycin |
| Vector Type | Expression Vector |
| Vector Size | 4488 bp |
The pMV261 vector is a widely used tool in molecular biology research. It is a plasmid vector that is commonly used for gene cloning and expression studies in bacterial systems.
One of the main applications of the pMV261 vector is gene cloning. This vector provides a suitable backbone for the insertion of foreign DNA sequences. Researchers can insert their gene of interest into the multiple cloning site (MCS) of the pMV261 vector, which contains a set of unique restriction enzyme sites.
Another important application of the pMV261 vector is gene expression studies. The vector contains a strong promoter sequence, which allows for efficient transcription of the inserted gene.
Moreover, the pMV261 vector can be used for protein expression studies. The vector contains a sequence that facilitates the production of recombinant proteins in bacteria. Researchers can insert their gene of interest into the vector, where it will be transcribed and translated into a protein of interest. This protein can then be purified and analyzed for its structure and function.
Overall, the pMV261 vector is a versatile tool in molecular biology research that allows for gene cloning, gene expression studies, and protein expression.
A: The promoter used in pMV261 is Hsp60.
A: pMV261 was described in the publication by Stover et al. in 1991, Nature 351:456.
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pMV261 is a high-copy number plasmid, which means that it can replicate in large quantities within the host cell. This is advantageous for obtaining high expression levels of the recombinant protein.
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