pHY300PLK

Name: pHY300PLK
SKU: VET1395
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : VET1395

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Product Information

Cat. No.	VET1395
Background	pHY300PLK is a shuttle vector that functions in Escherichia coli and Bacillus subtilis, which is constructed from the DNA of plasmid pACYC177 of Escherichia coli and plasmid pAMα1 derived from Streptococcus faecalis. This DNA can transform E. coli and Bacillus subtilis. pHY300PLK has ampicillin resistance gene and tetracycline resistance gene as selectable markers. This vector expresses both genes in E. coli but only the tetracycline resistance gene in B. subtilis.
Host Cell	Bacillus subtilis, Escherichia coli
Resistance	Ampicillin
Vector Type	Expression Vector
Vector Size	4870 bp

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Applications

Q & A

Customer Reviews

The pHY300PLK vector has found numerous applications in various fields of research. Some of the key applications include:

Gene Expression Studies: The pHY300PLK vector allows researchers to study gene expression by cloning the gene of interest into the vector and subsequently expressing it in a host organism. This enables the investigation of gene function, regulation, and biological pathways.

Protein Production: The pHY300PLK vector can be used for high-level expression of recombinant proteins in both prokaryotic and eukaryotic systems. This vector provides a robust and efficient system for large-scale production of proteins for biochemical, structural, and functional studies.

Molecular Cloning: The pHY300PLK vector serves as a versatile cloning tool for the construction of recombinant DNA molecules. It enables the straightforward insertion of DNA fragments into multiple cloning sites, making it an essential vector for routine cloning experiments.

Overall, the pHY300PLK vector is a versatile and widely used tool in molecular biology research.

Customer Q&As

What is pHY300PLK?

A: pHY300PLK is an Escherichia coli-Bacillus subtilis shuttle vector with ampicillin and tetracycline resistance genes.

What are the parental plasmids used in creating PHY300PLK?

A: The parental plasmids used in creating PHY300PLK are pACYC177 of E.coli and pAM-alpha of Streptococcus faecalis.

What genes are present in PHY300PLK?

A: PHY300PLK contains an RNA primer gene for ori-177, the R-Tc gene, the R-Ap gene, and the Rep-alpha-1 gene.

How many unique recognition restriction endonuclease sites have been found in pHY300PLK?

A: 23 unique recognition restriction endonuclease sites have been found in pHY300PLK.

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