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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Premade Virus Particles
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Premade AAV, adenovirus, lentivirus particles, safe, stable, in stock.
Virus-Like Particles (VLPs)
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Advanced VLPs for vaccine development (Chikungunya, Dengue, SARS-CoV-2), gene therapy (AAV1 & AAV9), and drug screening (SSTR2, CCR5).
Oligonucleotide Products
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Accelerate your research with cost-effective LncRNA qPCR Array Technology.
RNA Interference Products
Targeted | Potent | High Specificity
Human Druggable Genome siRNA Library enables efficient drug target screening.
Recombinant Drug Target Proteins
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Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
Clones
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Ready-to-use clones for streamlined research and development.
Kits
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Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
Enzymes
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Powerful Tn5 Transposase for DNA insertion and random library construction.
Aptamers
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Aptamers for key proteins like ACVR1A, Akt, EGFR, and VEGFR.
Adjuvants
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Enhance immune responses with high-purity, potent CpG ODNs.
Laboratory Equipment
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Effortlessly streamline DNA extraction with CB™ Magnetic-Nanoparticle Systems.
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Fast proposals, regular updates, and detailed reports; strict quality control, and contamination-free cells; knockout results in 4-6 weeks.
Target-based Drug Discovery Service
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Target identification, validation, and screening for drug discovery and therapeutic development.
Custom Viral Service
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Unbeatable pricing, fully customizable viral packaging services (covering 30,000+ human genes, 200+ mammals, 50+ protein tags).
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End-to-end antibody development support, from target to validation, enabling clients to rapidly obtain application-ready antibodies.
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Protein Degrader Service
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Harness the power of protein degraders for precise protein degradation, expanding druggable targets and enhancing therapeutic effectiveness for cutting-edge drug discovery.
Nucleotides Service
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Custom synthesis of oligonucleotides, primers, and probes for gene editing, PCR, and RNA studies.
Custom RNA Service
Custom RNA ServicePrecise | Flexible | GMP-ReadyCustom
RNA design, synthesis, and manufacturing—covering mRNA, saRNA, circRNA, and RNAi. Fast turnaround, rigorous QC, and seamless transition from research to GMP production.
Custom Libraries Construction Service
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Custom cDNA, genomic, and mutagenesis libraries for drug discovery, screening, and functional genomics.
Gene Editing Services
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Gene editing solutions for gene editing, knockouts, knock-ins, and customized genetic modifications. Integrated multi-platform solutions for one-stop CRISPR sgRNA library synthesis and gene screening services
Microbe Genome Editing Service
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Enhance microbial productivity with advanced genome editing using Rec-mediated recombination and CRISPR/Cas9 technologies.
Biosafety Testing Service
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Complete biosafety testing solutions for gene therapy, viral vectors, and biologics development.
Plant Genetic Modification Service
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Genetic modification for crop improvement, biotechnology, and plant-based research solutions.
Plant-based Protein Production Service
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Plant-based protein expression systems for biopharmaceuticals, enzyme production, and research.
Aptamers Service
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Revolutionizing drug delivery and diagnostic development with next-generation high-throughput aptamer selection and synthesis technologies.
CGT Biosafety Testing
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Internationally certified evaluation system for biologics, gene therapies, nucleic acid drugs, and vaccines.
Pandemic Detection Solutions
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Balancing accuracy, accessibility, affordability, and rapid detection to safeguard public health and strengthen global response to infectious diseases.
cGMP Cell Line Development
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Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
GMP mRNA Production
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Scalable mRNA production from milligrams to grams, with personalized process design for sequence optimization, cap selection, and nucleotide modifications, all in one service.
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Our plasmid production services span Non-GMP, GMP-Like, and GMP-Grade levels, with specialized options for linearized plasmids.
GMP Viral Vector Manufacturing
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Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
AI-Driven Gene Editing and Therapy
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AI-powered one-click design for customized CRISPR gene editing strategy development.
AI-Antibody Engineering Fusion
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AI and ML algorithms accelerate antibody screening and predict new structures, unlocking unprecedented possibilities in antibody engineering.
AI-Driven Enzyme Engineering
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High-throughput enzyme activity testing with proprietary datasets and deep learning models for standardized and precise enzyme engineering design.
AI-Enhanced Small Molecule Screening
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Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
AI-Driven Protein Degrader Drug Development
Innovative | Targeted | Accelerated
Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
Jasmonate (JA) is an important hormone that plays a role in plant defense. The cam1 and wrky53 mutants were more resistant to Spodoptera litura than the wild-type (WT) Arabidopsis group. Furthermore, the JA concentration was higher in cam1 and wrky53 mutants compared with the WT group. To explore how these two proteins influence resistance in Arabidopsis plants, the researchers used a yeast two-hybrid assay, firefly luciferase complementation imaging assay and in vitro pull-down assay confirming that calmodulin 1 (CAM1) interacted with WRKY53. This study shows that CAM1 and WRKY53 regulate the JA biosynthetic pathway through CAM1-WRKY53 dissociation, thereby negatively regulating plant resistance to herbivory, and then the released WRKY53 binds to the LOXs promoter and negatively regulates LOXs gene expression. This study reveals the insect-resistant mechanism of WRKY53 and provides new clues to the function of WRKY53.
In luciferase activity assays, WRKY53 pGreenII 62-SK interacted with the LOX3 promoter pGreenII 0800-LUC, and WRKY53 pGreenII 62-SK negatively regulated LOX3 pGreenII 0800-LUC expression. However, when CAM1 pGreenII 62-SK was added to the system, LOX3 pGreenII 0800-LUC expression returned to control levels.
Figure 1. WRKY53 pGreenII 62-SK negatively affects LOX3 expression, and CAM1 pGreenII 62-SK decreased the negative regulation. (Jiao, Chunyang, et al., 2022)
A: The size of the pGreenII 62-SK vector is 3347 bp.
A: DH5α, TOP10, XL1-Blue, and similar strains can be used.
A: The recommended working concentration is 25μg/ml.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The carrier provided by the company underwent strict quality control.
United States
05/05/2023
The pGreenII 62-SK vector provides a good amount of capacity for DNA inserts. This allowed me to insert the DNA sequences that I needed without worrying about the vector's capacity constraints.
Canada
01/09/2020
Given pGreenII 62-SK vector's high performance and the range of features it provides, I found it to be quite affordable compared to other similar products in the market.
United States
10/04/2023
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