pCoHygro

pCoHygro, or the pCoHygro plasmid, is a commonly used plasmid in molecular biology research. Here are some applications of pCoHygro: 1. Generation of stable cell lines: pCoHygro can be used to generate stable cell lines expressing a gene of interest. The plasmid contains a selectable marker gene (hygromycin resistance) which allows for the identification and maintenance of cells that have successfully incorporated the plasmid. 2. Gene expression studies: pCoHygro can be used to study gene expression by introducing a promoter sequence upstream of the gene of interest. This allows researchers to investigate the effects of specific promoters on gene expression in different cellular contexts. 3. Protein localization studies: By fusing a fluorescent protein or epitope tag to the gene of interest in pCoHygro, researchers can investigate the subcellular localization of the protein within cells. 4. Functional analysis of genes: pCoHygro can be used to create gene knockout or knockdown cell lines, allowing researchers to study the function of specific genes by observing the phenotypic consequences of their loss. 5. Recombinant protein production: pCoHygro can be used to express and produce recombinant proteins of interest in cells. The selectable marker gene ensures the maintenance of cells with the plasmid, allowing for long-term protein production.