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pCHO1.0

For research use only. Not intended for any clinical use.
Cat.No.
VET1393
Host Cell
mammalian cells, Escherichia coli
Promoter
CMV
Resistance
Kanamycin
Selection
Puromycin
Vector Size
12988 bp
Vector Type
Expression Vector

Background

Applications

Publications

Q & A

Customer Reviews

The pCHO 1.0 vector is aimed at expressing either a single gene or co-expressing two target genes facilitated by the hybrid CMV promoter presented on the vector. This vector bears the dihydrofolate reductase (DHFR) selective marker and the puromycin resistance gene, thus allowing for simultaneous screening using both MTX and puromycin. Recognized as a mammalian expression vector, the pCHO 1.0 vector adeptly employs the CMV promoter, renowned for its high expression levels. This vector adheres to the multicloning site method and restricts endonuclease for efficient cloning. It is considerably large, with a total size of 12,988 base pairs. This vector possesses resistance against kanamycin, an antibiotic often used in molecular biology, which allows for easier selection of successfully transfected cells. Moreover, for selection, the vector integrates two distinct markers. The first one is puromycin, an antibiotic that inhibits protein synthesis in a wide variety of organisms. The second one is DHFR, an enzyme involved in the folate metabolism pathway, whose high level expression can be used for selection in various cell types.
The pCHO1.0 vector is a powerful tool used in molecular biology and biotechnology, with a range of applications including: Protein Production: The pCHO1.0 vector is commonly used in bioengineering to produce large quantities of specific proteins. This is often done in Chinese Hamster Ovary (CHO) cells, a popular cell line used in biomanufacturing due to its ability to correctly fold and modify human proteins. Monoclonal Antibody Production: The pCHO1.0 vector can also be used to produce monoclonal antibodies. These are identical immune cells that are clones of a unique parent cell, primarily used for the detection of specific proteins. Study of Gene Function: The pCHO1.0 vector can be used to overexpress a gene of interest in order to study its function. This could be important in fundamental biological research and in identifying potential drug targets. Cell Line Development: The pCHO1.0 vector can be used to create stable cell lines. These are genetically identical cells that are able to proliferate indefinitely. They are used in a vast array of biological research and industrial applications, from drug development to genetic engineering. Recombinant Protein Production: Recombinant proteins are proteins that are derived from one gene and placed into another organism. The pCHO1.0 vector can insert specific genes into CHO cells to produce those proteins. Therapeutic Applications: The pCHO1.0 vector can be used to produce proteins for therapeutic applications. For instance, they are used for the production of biologics, a class of drugs that include vaccines, blood components, allergenics, somatic cells, gene therapies, tissues, and recombinant therapeutic proteins.
Customer Q&As
What are the capabilities of the pCHO 1.0 vector?

A: The pCHO 1.0 vector can express one gene or co-express two target genes using the hybrid CMV promoter on the vector.

Which selective markers are included in the pCHO 1.0 vector?

A: The pCHO 1.0 vector contains the dihydrofolate reductase (DHFR) selective marker and the puromycin resistance gene.

Can the pCHO 1.0 vector be used for dual selection with both methotrexate (MTX) and puromycin?

A: Yes, the pCHO 1.0 vector allows for simultaneous selection using both MTX and puromycin.

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Customer Reviews
Less time-consuming

One of the most admirable features of the pCHO1.0 vector is its straightforward cloning capacity. This vector enables the easy integration of genetic material, making the task more convenient and less time-consuming for us.

United Kingdom

10/14/2021

The pCHO1.0 vector's stability is remarkable.

The pCHO1.0 vector's stability is remarkable. It ensures the persistent expression of Our protein of interest, which is crucial for long-term studies.

Germany

05/15/2021

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