Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1185
| Cat. No. | VET1185 |
| Species | Bacterial |
| Tag | C-His, C-V5 |
| Source | Saccharomyces cerevisiae, Escherichia coli |
| Promoter | GAL1 |
| Selection | Ampicillin, Blasticidin |
A: In yeast, high copies of the plasmids were maintained by using the 2 μ replicon in the vector.
A: The pYES6/CT vector contains the following elements: 1. Yeast GAL 1 promoter.2. Multiple cloning sites.3. Ampicyl resistance gene.4. The isin resistance gene.5 The C terminus of the vector encodes the V5 epitope and the His purified label. The 6.2- μ replicon.
A: The yeast GAL1 promoter, is able to express target proteins with high levels of inducible protein by galactose in S. cerevisiae, while being able to suppress expression by glucose.
A: Multicloning sites can use 10 restriction sites,usually we can ues two BstX I restriction sites.
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The vector has 10 restriction sites can be used, and two BstX I restriction sites to facilitate gene insertion.
The ampicyl resistance gene on this vector can facilitate vector screening in E. coli.
Incidin (Blasticidin) resistance gene can facilitate vector screening in Escherichia coli and yeast.
The C terminus of the vector encodes the V5 epitope and a His purification tag to facilitate protein purification and western blot detection.
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