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pYES6/CT

For research use only. Not intended for any clinical use.
Cat.No.
VET1185
Promoter
GAL1
Selection
Ampicillin, Blasticidin
Source
Saccharomyces cerevisiae, Escherichia coli
Species
Bacterial
Tag
C-His, C-V5

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Q & A

Customer Reviews

Customer Q&As
How does the vector maintain a high copy plasmid in yeast?

A: In yeast, high copies of the plasmids were maintained by using the 2 μ replicon in the vector.

What elements does pYES6/CT contain?

A: The pYES6/CT vector contains the following elements: 1. Yeast GAL 1 promoter.2. Multiple cloning sites.3. Ampicyl resistance gene.4. The isin resistance gene.5 The C terminus of the vector encodes the V5 epitope and the His purified label. The 6.2- μ replicon.

What does the yeast GAL1 promoter on the pYES6/CT vector do?

A: The yeast GAL1 promoter, is able to express target proteins with high levels of inducible protein by galactose in S. cerevisiae, while being able to suppress expression by glucose.

How many restriction sites can be used for the polycloning site on this vector?

A: Multicloning sites can use 10 restriction sites,usually we can ues two BstX I restriction sites.

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Customer Reviews
Worked Very Well

The vector has 10 restriction sites can be used, and two BstX I restriction sites to facilitate gene insertion.

United States

04/11/2021

Easy to Use

The ampicyl resistance gene on this vector can facilitate vector screening in E. coli.

United States

11/21/2021

Facilitate vector screening

Incidin (Blasticidin) resistance gene can facilitate vector screening in Escherichia coli and yeast.

United States

01/31/2022

Facilitate protein purification and western blot detection

The C terminus of the vector encodes the V5 epitope and a His purification tag to facilitate protein purification and western blot detection.

United States

08/05/2022

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