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pTYB12

For research use only. Not intended for any clinical use.
Cat.No.
VET1178
Promoter
T7
Selection
Ampicillin
Source
Escherichia coli
Species
Bacterial

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Q & A

Customer Reviews

Customer Q&As
What is the function of the autocleavage activity of this internal protein?

A: The self-cleavage activity of the intein allows the release of the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein. 

Why can the N-terminus of the target protein on this vector be fused to the intein tag?

A: It is a N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker, downstream of the intein tag. This allows the N-terminus of the target protein to be fused to the intein tag.

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Customer Reviews
This vector can be used in conjuction with pTYB2 (NEB #N6702S) to test which fusion construction (N-terminal or C-terminal) maximizes the expression and yield of a target protein.

This pQBI63 vector can be used in conjuction with pTYB2 to test which fusion construction maximizes the expression and yield of a target protein.

United States

11/21/2021

Convenient

pTYB12 is an E. coli cloning and expression vector used in the Protein Purification System which allows the overexpression of a target protein as a fusion to a self-cleavable affinity tag,which is very convenient.

United States

01/31/2022

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