Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1011
| Cat. No. | VET1011 |
| Species | Bacterial |
| Tag | N-His |
| Source | Escherichia coli |
| Promoter | T5 |
| Selection | Ampicillin |
A: For TAGZyme cleavage, a glutamine should be inserted at an uneven amino acid position, followed by the first amino acid of the expressed protein.
A: Lac promoter: It comes from the lactose operon of E. coli. It is a directional nucleotide sequence on a DNA molecule and consists of the repressor protein gene (LacI), initiator gene (P), manipulation gene (O) and the gene structure encoding three enzymes involved in lactose utilization
A: The Lac promoter is positively regulated by the catabolic system and negatively regulated by repressors. Positive regulation activates the promoter through the CAP (catabolite gene activation protein) factor and cAMP to promote transcription. Negative regulation is the production of the LacZ repressor protein, which binds to the manipulated gene to prevent transcription.
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The Vector is recommended by several colleagues and are easy to perform and reliable, saving a lot of time in laboratory operations.
The Vector used in our laboratory is this type, high efficiency, many positive clones.
The plasmid is very good and it has a relatively high conversion efficiency,I will be used for the future.
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