pMBP-P

Name: pMBP-P
SKU: VET1168

For research use only. Not intended for any clinical use.

Cat.No.

VET1168

Promoter

Tac/lac

Selection

Ampicillin

Source

Escherichia coli

Species

Bacterial

Tag

N-MBP, N-Thrombin, C-6×His

Publications

Q & A

Customer Reviews

Customer Q&As

what type of plasmid is the pMBP-P plasmid? How to distinguish tight control and relaxed control plasmid?

A: The replicase lines of replicative plasmids share with chromosomal DNA replication and can only be replicated at a certain stage of the cell cycle. When the cell chromosome stops replication, the plasmid will no longer replicate. The replicase line of the relaxed control replicative type is unaffected by the chromosomal DNA replicase line, can replicate at any time throughout the cell growth cycle, and the plasmid can continue when chromosomal replication has ceased.

What does pMBP-P plasmid incompatibility mean?

A: Incompatibility refers to the phenomenon that structurally similar and closely related plasmids do not stably coexist within the same host bacteria, and vice versa.

What can the pMBP-P plasmid compatibility and insolubility be used for?

A: The compatibility and insolubility of plasmids are often used in epidemiological investigations.

Are pMBP-P plasmids with different genens sizes easy to extract?

A: Different plasmids range between 2 and 300 kb, and small plasmids smaller than 15 kb are easier to separate and purify, while large plasmids larger than 15 kb are not easy to extract.

Does the gene on the pMBP-P vector need to be induced for expression?

A: The pMBP-P vector needs to be induced by IPTG for expression in BL21 (DE3), with a plasmid size of 6741bp and the replicon of ColE1 ori, a high-copy plasmid.

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