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A: The MBP in these vectors has been engineered for tighter binding to amylose. The method uses the strong “tac” promoter and the malE translation initiation signals to give high-level expression of the cloned sequences, and a one-step purification of the fusion protein using MBP’s affinity for maltose
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
We used this vector to attach a mbp tag to the gene, which can well contribute to protein expression.
United States
03/31/2023
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