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pIEXBac-1

For research use only. Not intended for any clinical use.
Cat.No.
VET1237
Promoter
IE1, p10
Selection
Ampicillin
Source
Insect cell, Escherichia coli
Species
Bacterial
Tag
C-His,N-Strep

Publications

Q & A

Customer Reviews

Customer Q&As
How is the dual-purpose vector designed to clone and express proteins at high levels?

A: se pIExBac vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants.

How do genes on pIEx / Bac vectors achieve high-level expression of proteins?

A: The pIEx/Bac-1 vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants.

What promoter on the pIEXBac-1 vector drives late/very late expression in baculovirus mode?

A: Late/very late expression in the baculovirus mode is driven by the strong p10 promoter.

Which sites are carried by the N-terminus of the pIExBac-1 vector?

A: The pIEx/Bac-1 vector carries an N-terminal Strep Tag II coding sequence followed by a recognition site for enterokinase.

Why does the pIEXBac-1 vector carry two tags?

A: The presence of two tags is ideal for dual purification strategies designed to isolate full-length fusion proteins.

How does the pIEXBac-1 vector achieve the isolation of the full-length fusion proteins?

A: Two “gentle elution" tags at both the N- and C-terminus is helpful for isolate full-length fusion proteins

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Customer Reviews
High-level expression of proteins

The dual-purpose pIEx/Bac vectors are designed for cloning and high-level expression of proteins.we can transiently transfect Spodoptera-derived insect cells or by generating baculovirus recombinants.

United States

08/23/2020

A deal choice

The multiple cloning region of pIEXBac-1 vector is followed by an optional C-terminal His•Tag coding sequence, which is a deal choice.

United States

05/12/2021

Dual purification

The pIEXBac-1 vector is ideal for dual purification strategies designed to isolate full-length fusion proteins.

United Kingdom

08/15/2021

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