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pGAPZB

For research use only. Not intended for any clinical use.
Cat.No.
VET1192
Promoter
GAP
Selection
Zeocin
Source
Pichia pastoris, Escherichia coli
Species
Bacterial
Tag
C-Myc, C-His

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Q & A

Customer Reviews

Customer Q&As
Which tags are there on the vector that we can use?

A: The recombinant proteins were expressed as fusion proteins with a C-terminal myc antigen epitope and a C-terminal His tag. In addition, the fusion protein expressed by pGAPZα secreted a signal peptide at the N-terminal with the α -factor of S. cerevisiae.

What are the characteristics of the pGAPZB?

A: The pGAPZB provides three different read frames to facilitate cloning in vectors with C-terminal tags and / or N-terminal secreted signal peptide.

What selection markers are the pGAPZB based on?

A: The pGAPZB vectors are based on the dominant selection markers, and the Zeocin (bleomycin) screening markers can be used in both Pichia pastoris and Escherichia coli.

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Customer Reviews
Stably expressed

The pGAPZB vectors stably expressed the recombinant protein in Pichia yeast using the GAP promoter.

United States

04/06/2023

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