Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1192
| Cat. No. | VET1192 |
| Species | Bacterial |
| Tag | C-Myc, C-His |
| Source | Pichia pastoris, Escherichia coli |
| Promoter | GAP |
| Selection | Zeocin |
A: The recombinant proteins were expressed as fusion proteins with a C-terminal myc antigen epitope and a C-terminal His tag. In addition, the fusion protein expressed by pGAPZα secreted a signal peptide at the N-terminal with the α -factor of S. cerevisiae.
A: The pGAPZB provides three different read frames to facilitate cloning in vectors with C-terminal tags and / or N-terminal secreted signal peptide.
A: The pGAPZB vectors are based on the dominant selection markers, and the Zeocin (bleomycin) screening markers can be used in both Pichia pastoris and Escherichia coli.
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The pGAPZB vectors stably expressed the recombinant protein in Pichia yeast using the GAP promoter.
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