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A: The expression vector of Pichia yeast is an integrated plasmid, which contains the sequence homologous to the yeast chromosome.
A: Some Picranias are histidine defective, such as GS115, which cannot synthesize histidine by itself and must be supplemented with histidine in the culture medium to grow (natural media such as YPD itself contains histidine, MM or MD without addition). Some vectors contain the HIS 4 gene, and when integrated into the yeast genome, the hisis 4 gene can synthesize histidine. Therefore, if it is a vector containing the His4 gene, this feature can be used to screen, and the plate without histidine, usually the MD plate.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Pichia yeast expression system is the most widely used yeast expression system at present. The expression vector of Pichia yeast is an integrated plasmid,so it is easy to integrate into the yeast chromosome.
United States
03/15/2023
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