Pages
Products

pFastBacHT B

For research use only. Not intended for any clinical use.
Cat.No.
VET1231
Promoter
Polyhedrin
Selection
Ampicillin, Gentamicin
Source
Insect cell, Escherichia coli
Species
Bacterial
Tag
N-His

Publications

Q & A

Customer Reviews

Customer Q&As
How does the pFastBacHT B vector drive the GST pro-soluble tag to ensure the target gene fusion expression?

A: The pFastBacHTB plasmid sequence is an E. coli expression vector, and the strong Tac promoter can drive GST lytic tag and target gene fusion expression.

What is the role of LacI in blocking the protein and Laco operator?

A: LacI blocking protein and Laco operator allow the essential particle to prohibit expression before adding IPTG, preventing it from affecting bacterial growth.

How does the expressed fusion protein remove the GST tag?

A: The expressed fusion protein can be cleaved by thrombin protease, and the GST tag can be removed from the GST column again.

What is the purpose of this pFastBacHT B vector?

A: Plasmid usage: protein expression; gene editing; induced staining; location hybridization, etc.

What hosts are available for this pFastBacHT B plasmid?

A: Plasmid host: Escherichia coli; mammalian cells; yeast; phage; Lactobacillus; Bacillus subtilis; filamentous fungi; Staphylococcus aureus, etc.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Customer Reviews
Efficient

The strong Tac promoter of this vector can drive GST lytic tag and target gene fusion expression,very efficient.

United States

01/27/2022

Will not affect the bacterial growth

The vector will not affect the bacterial growth,since LacI blocking protein and Laco operator allow the essential particle to prohibit expression before adding IPTG.

United States

07/19/2022

Simple operation

The operation is very simple,the expressed fusion protein we want to express can be cleaved by thrombin protease, and the GST tag can be removed from the GST column again.

France

11/03/2022

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

CBpromise

Our promise to you:
Guaranteed product quality, expert customer support.

24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Quick Inquiry

Inquiry