Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1231
| Cat. No. | VET1231 |
| Species | Bacterial |
| Tag | N-His |
| Source | Insect cell, Escherichia coli |
| Promoter | Polyhedrin |
| Selection | Ampicillin, Gentamicin |
A: The pFastBacHTB plasmid sequence is an E. coli expression vector, and the strong Tac promoter can drive GST lytic tag and target gene fusion expression.
A: LacI blocking protein and Laco operator allow the essential particle to prohibit expression before adding IPTG, preventing it from affecting bacterial growth.
A: The expressed fusion protein can be cleaved by thrombin protease, and the GST tag can be removed from the GST column again.
A: Plasmid usage: protein expression; gene editing; induced staining; location hybridization, etc.
A: Plasmid host: Escherichia coli; mammalian cells; yeast; phage; Lactobacillus; Bacillus subtilis; filamentous fungi; Staphylococcus aureus, etc.
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The strong Tac promoter of this vector can drive GST lytic tag and target gene fusion expression,very efficient.
The vector will not affect the bacterial growth,since LacI blocking protein and Laco operator allow the essential particle to prohibit expression before adding IPTG.
The operation is very simple,the expressed fusion protein we want to express can be cleaved by thrombin protease, and the GST tag can be removed from the GST column again.
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