pFastBacDual

Name: pFastBacDual
SKU: VET1233
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : VET1233

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Product Information

Cat. No.	VET1233
Species	Bacterial
Tag	N-His, N-TEV
Source	Insect cell, Escherichia coli
Promoter	Polyhedrin, P10
Selection	Ampicillin, Gentamicin

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Background

Applications

Q & A

Customer Reviews

Pfastbac dual insect cell expression vector, commonly known as pfastbac dual, is a specialized vector designed to express two proteins simultaneously in insect cells via the BAC to BAC expression system. The unique feature that differentiates this vector from others on the market is its two promoters, i.e., the Polyhedrin (PH) promoter and the P10 promoter.

The Polyhedrin promoter and the P10 promoter, present in the pFastBacDual vector, facilitate high-level protein expression. This vector is of a considerable size, approximately 5238bp, and is characterized by a PUC replicator and an SV40 poly (a) signal terminator. When it comes to resistance, the vector hosts prokaryotic resistance to amp and eukaryotic resistance to gen. The plasmid host in general for pFastBacDual is an insect cell and its chief purpose is protein expression. The efficiency of the pFastBacDual vector makes it a popular choice among researchers in the field of protein-based studies.

The pFastBacDual vector is used for generating recombinant baculoviruses for insect cell expression. Here are some of its applications:

Protein Expression: This vector has two multiple cloning sites which can be used to simultaneously express two different genes in insect cells, which will facilitate protein structure and function study.

Proteomics Research: It helps researchers to study protein interactions and modifications, and protein localization within cells.

High-throughput Screening: With its capability to produce large amounts of proteins, this vector is suitable for high-throughput screening applications.

Vaccine Production: The pFastBacDual vector can be used to develop and produce vaccines. Through recombinant baculovirus, large amount of antigens can be produced in insect cells, and these antigens can be used as the component of vaccine.

Biologic Drugs Production: The pFastBacDual vector enables the production of biologic drugs in insect cells. The vector system allows for effective protein folding and post-translational modifications, which are crucial for the functionality of many biologic drugs.

Customer Q&As

Can pFastBac Dual vectors express both proteins simultaneously in insect cells?

A: The pFastBa Dual vector is characterized by two promoters on a single vector used to express both proteins in insect cells while using the Bac-to-Bac baculovirus expression system.

Why can pFastBac Dual vectors achieve high levels of expression?

A: The vector has two high promoters, the polyheme protein promoter and the p10 promoter and therefore achieves high levels of expression.

What is the purpose of the pFastBac Dual vector?

A: The pFastBac Dual vector is used for efficient gene transfer and coexpression of two target proteins in mammalian cells.

How many multiple cloning sites does the pFastBac Dual vector have?

A: The pFastBac Dual vector contains two multiple cloning sites.

Which promoters control the expression of the two heterologous genes in the pFastBac Dual vector?

A: One gene is controlled by the PH promoter, and the other is controlled by the p10 promoter.

What is required for proper expression of recombinant proteins in the pFastBac Dual vector?

A: The inserts cloned into the pFastBac Dual vector must contain an ATG start codon for translation initiation and a stop codon for gene termination.

What type of proteins are produced by the pFastBac Dual vector?

A: The pFastBac Dual vector produces non-fusion proteins.

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Customer Reviews

Convenient and concise

The pFastBac Dual vector has two promoters on a single vector, so we can use this vector to express both proteins simultaneously in insect cells. Convenient and concise.

United States

2023-02-23

High level expression

This pFastBacDual vector has two strong promoters, the polyheome protein promoter and the p10 promoter, so we used this vector to achieve protein high level expression.

United States

2023-04-11

Greatly facilitate our workflow

The pFastBacDual vector has proven to be an essential part of our research. It enables us to express and study two different genes at the same time which greatly facilitates our workflow.

United States

2022-05-28

An excellent choice

Efficiency and reliability are the key aspects I take into consideration when choosing any vector. The Creative Biogene's pFastBacDual vector meets these criteria and does not disappoint.

United States

2023-06-11

Repeatable results

I have been utilizing the pFastBacDual vector for some time now and it has consistently provided reliable and repeatable results.

French

2021-09-05

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pFastBacDual

Product Information

Background

Applications

Q & A

Customer Reviews

Can pFastBac Dual vectors express both proteins simultaneously in insect cells?

Why can pFastBac Dual vectors achieve high levels of expression?

What is the purpose of the pFastBac Dual vector?

How many multiple cloning sites does the pFastBac Dual vector have?

Which promoters control the expression of the two heterologous genes in the pFastBac Dual vector?

What is required for proper expression of recombinant proteins in the pFastBac Dual vector?

What type of proteins are produced by the pFastBac Dual vector?

Convenient and concise

High level expression

Greatly facilitate our workflow

An excellent choice

Repeatable results

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