pEF1/myc-His B

Name: pEF1/myc-His B
SKU: VET1242

For research use only. Not intended for any clinical use.

Cat.No.

VET1242

Promoter

EF-1a

Selection

Ampicillin, Neomycin

Source

Insect cell, Escherichia coli

Species

Bacterial

Tag

6X His, myc

Publications

Q & A

Customer Reviews

Customer Q&As

In which cell lines can the vector epitope replicate?

A: Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen.

What experiments can the vector be used for control?

A: The control plasmid pEF1/myc-His lacZ was used as a positive control and could be used during transfection, expression and detection of selected cell lines.

What cell lines is the pEF1/myc-His B vector designed to be used in?

A: pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines.

In what cells can the pEF1/myc-His B vector undergo high levels of stable and transient expression?

A: High-level stable and transient expression can be carried out in most mammalian cells.

Why can the pEF1/myc-His B vector be expressed at high levels in a wide range of species and cell types?

A: Human elongation factor 1-subunit promoter for high-level expression across a broad range of species and cell types.

What are the roles of the three reading frames on this carrier?

A: Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide.

What resistance was selected for this pEF1/myc-His B vector to select for stable cell lines?

A: Neomycin resistance gene for selection of stable cell lines.

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