Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1250
| Cat. No. | VET1250 |
| Species | Bacterial |
| Tag | N-His, N-Trigger factor, N-HRV 3C, N-Thrombin, N-Factor Xa |
| Source | Escherichia coli |
| Promoter | CSPA |
| Selection | Ampicillin |
A: HRV 3C protease, thrombin, and Factor Xa recognition sites are located between "Trigger Factor" and the polyclonal site (MCS) to remove the tags from the expressed fusion protein.
A: Most E. coli strains can be used as the expression host for this vector.
A: The pCold-TF vector provides a cold shock-induced method for high-level expression of target proteins, coupled with the expression of the trigger factor (chaperone) to improve correct protein folding, thus achieving efficient soluble expression of refractory proteins in other systems.
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