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pCold-TF

For research use only. Not intended for any clinical use.
Cat.No.
VET1250
Promoter
CSPA
Selection
Ampicillin
Source
Escherichia coli
Species
Bacterial
Tag
N-His, N-Trigger factor, N-HRV 3C, N-Thrombin, N-Factor Xa

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Customer Q&As
How does the vector remove the tag on the expressed fusion protein?

A: HRV 3C protease, thrombin, and Factor Xa recognition sites are located between "Trigger Factor" and the polyclonal site (MCS) to remove the tags from the expressed fusion protein.

What are the expression hosts of pCold-TF?

A: Most E. coli strains can be used as the expression host for this vector.

How does this vector achieve efficient and soluble expression of intractable proteins in other systems?

A: The pCold-TF vector provides a cold shock-induced method for high-level expression of target proteins, coupled with the expression of the trigger factor (chaperone) to improve correct protein folding, thus achieving efficient soluble expression of refractory proteins in other systems.

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