Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1153
| Cat. No. | VET1153 |
| Species | Bacterial |
| Tag | N-His, N-Factor Xa |
| Source | Escherichia coli |
| Promoter | CSPA |
| Selection | Ampicillin |
A: When the temperature of cultured E. coli is sufficiently reduced, almost temporarily stopped growth most protein expression is reduced, when a group of proteins called "cold shock proteins" are specifically induced.
A: The cold shock expression vector, pCold I-IV, is a high-efficiency protein expression vector designed using the cold shock gene CSPA promoter.
A: Downstream of the promoter of CSPA, the lac operon was inserted to allow for tight control of expression.
A: There are four types of pCold vectors with slightly different positions, TEE, His tag sequence and Factor XA restriction sites.
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