pBlueScript SK(+) vector

pBluescript SK(+) is a high copy number standard cloning vector commonly used in molecular biology. With a length of 2958 bp, it is developed and utilized for cloning, sequencing, and expression studies. pBluescript SK(+) is recognized for its resistance to the antibiotic Ampicillin, which means that this vector can be used to transform bacteria that are susceptible to Ampicillin. It usually has a resistance gene located in its backbone that can express a protein to neutralize the lethal effects of this type of antibiotic. This feature plays a significant role in the selection of successfully transformed cells. Interestingly, the f1 (+) orientation of pBluescript SK(+) provides a rescue mechanism for sense strand ssDNA, which is vital for overcoming challenges associated with single-strand DNA instability. Furthermore, pBluescript SK(+) has a reversed multiple cloning site (MCS), a short segment containing several commonly used restriction enzyme recognition sites which allow insertion of DNA fragments at this location. This feature greatly increases the versatility of the vector as it allows the insertion of foreign DNA at several unique restriction sites, enabling a variety of manipulation techniques.