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pAO815

For research use only. Not intended for any clinical use.
Cat.No.
VET1194
Promoter
AOX1
Selection
Ampicillin, HIS4
Source
Pichia pastoris, Escherichia coli
Species
Bacterial

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Q & A

Customer Reviews

Customer Q&As
What special operations are required when using this vector?

A: For vector construction, it needs to contain an ATG promoter, along with the addition of the Kozak sequence.

What restriction enzymes are needed to insert a gene into the HIS4 of GS115 and KM71 of the vector?

A: The insertion of genes into the HIS4 of GS115 and KM71 requires the use of either a SalI or a Stu I linearized plasmid.

What is the yeast genotype produced by inserting the gene into the AOX 1 gene of GS115?

A: Insertion of the gene into the AOX1 gene of GS115 could use the BglII restriction enzyme linearized plasmid with the resulting yeast genotype of His+ MutS.

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