Cat.No. : CSC-RR0541 Host Cell: HEK293T
| Cat. No. | CSC-RR0541 |
| Description | This cell line is engineered to co-express human KDR and luciferase reporter gene under the control of NFAT response element. |
| Gene | NFAT-Luc/KDR |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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We have been using the NFAT-Luc/KDR Stable Cell Line - HEK293T for our angiogenesis studies and its performance has been excellent. The luciferase reporter activity is consistent and reliable, providing us with reproducible results across multiple experiments.
We have tested various cell lines for our assays, and the NFAT-Luc/KDR Stable Cell Line - HEK293T stood out for its superior sensitivity in detecting NFAT-driven luciferase activity.
This stable cell line has greatly accelerated our research timeline. The ready-to-use nature of these cells has streamlined our workflow, allowing us to focus more on data analysis rather than cell line preparation and maintenance.
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