Transfected Stable Cell Lines
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Cat. No. : CSC-RR0461
Host Cell : Colon26 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0461 |
| Description | Colon26-Luc cell line is engineered to stably overexpress luciferase under a constitutive promoter. It is an ideal positive control for use in bioluminescence assay. |
| Host Cell | Colon26 |
| Host Cell Species | Mus musculus (Mouse) |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Colon-26 is a mouse colon adenocarcinoma cell line derived from the tumor tissue of Balb/c mice bearing Colon-26 carcinoma, induced by a single rectal application of N-Nitroso-N-Methyl-urethan (NMU). Colon-26 cells are used to create a mouse model of cachexia for the study of cancer cachexia, a syndrome of progressive loss of skeletal muscle mass and fat tissue that results in weight loss and weakness. Additionally, the colon-26 cell line can be used for metastasis studies. Injection of the colon-26 cell line into the spleen of wild-type mice induces tumor necrosis factor alpha (TNF-α) expression and subsequent liver metastasis formation, which may be mediated by tumor necrosis factor receptor p55 signaling.
Luciferase Stable Cell Line - Colon26 is a genetically engineered cell line modified to express luciferase. The introduction of the luciferase gene into the Colon26 cell line allows measurement of cellular activity through luminescence. This is particularly useful in the field of biomedical research, where it is often used in in vivo imaging studies to track tumor growth and metastasis, monitor gene expression, or evaluate the effectiveness of therapeutic interventions.
The luciferase stable cell line-Colon26 is mainly used in the field of biomedical research, especially research related to colon cancer. Here are the applications:
Live cell imaging: Bioluminescent signals from luciferase enable real-time monitoring of biological processes.
Drug screening: This cell line may serve as a useful model for high-throughput screening of potential therapeutic compounds for colon cancer. By monitoring changes in bioluminescence, researchers can assess how effectively the drug inhibits cancer cell growth.
Gene regulation studies: This cell line can be used to study gene expression in colon cancer. By inserting a promoter sequence upstream of the luciferase gene, the expression of this specific gene can be monitored based on the bioluminescence signal.
Tumor growth and metastasis studies: Luciferase-stable cell lines are commonly used in mouse models to study tumor growth and metastasis. Injecting these cells into mice and observing the bioluminescent signal allowed researchers to track tumor growth, spread and metastasis in the body.
Testing treatment strategies: Researchers used this cell line to validate the efficacy of various treatment strategies against colon cancer cells in vitro and in vivo.
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The GFP Stable Cell Line - MIA PaCa-2 has been an invaluable addition to our research toolkit. The cells' consistent and robust GFP expression allows for easy visualization and tracking, greatly simplifying our experimental workflows.
The bright and stable GFP signal remains unaltered over multiple passages, making long-term studies feasible and accurate. This product has exceeded our expectations.
The cells adapt well to various experimental conditions and maintain a strong GFP expression throughout. This consistency has been crucial for the success of our fluorescence-based assays and in vivo studies.
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