Cat.No. : CSC-RR0461 Host Cell: Colon26
| Cat. No. | CSC-RR0461 |
| Description | Colon26-Luc cell line is engineered to stably overexpress luciferase under a constitutive promoter. It is an ideal positive control for use in bioluminescence assay. |
| Host Cell | Colon26 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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The GFP Stable Cell Line - MIA PaCa-2 has been an invaluable addition to our research toolkit. The cells' consistent and robust GFP expression allows for easy visualization and tracking, greatly simplifying our experimental workflows.
The bright and stable GFP signal remains unaltered over multiple passages, making long-term studies feasible and accurate. This product has exceeded our expectations.
The cells adapt well to various experimental conditions and maintain a strong GFP expression throughout. This consistency has been crucial for the success of our fluorescence-based assays and in vivo studies.
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