Cat.No. : CSC-RO1122 Host Cell: A549
| Cat. No. | CSC-RO1122 |
| Description | This cell line is engineered to stably overexpress human VHL in A549 cells. |
| Gene | VHL |
| Gene Species | Homo sapiens (Human) |
| Host Cell | A549 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line, by stably expressing the VHL gene, provides a controllable experimental model for studying how the VHL protein functions as a tumor suppressor, helping to reveal key mechanisms of VHL in regulating cell proliferation, apoptosis, and hypoxia response.
A: By culturing A549 cells under hypoxic conditions, researchers can observe the impact of VHL gene expression on HIF stability and activity, thereby understanding the role of VHL in the hypoxia signaling pathway.
A: This cell line can be used to evaluate the effects of potential therapeutic compounds on VHL function, especially in VHL-related diseases such as Von Hippel-Lindau disease, aiding in the screening of drugs that can restore normal VHL function.
A: By monitoring the relationship between VHL expression levels and cell cycle progression, researchers can explore the role of VHL in cell cycle regulation, including its effects on cyclins and cyclin-dependent kinases.
A: This cell line can be used to study the role of the VHL gene in regulating apoptosis, especially the changes in apoptotic pathways when VHL function is lost.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Human VHL Stable Cell Line - A549 uses precise genetic engineering techniques to stably express the VHL gene in A549 cells, providing a highly controlled environment for studying the function of VHL protein.
By utilizing the Human VHL Stable Cell Line - A549, researchers can better understand the role of the VHL gene in the initiation and progression of tumors, aiding in the development of targeted cancer treatment strategies.
This product ensures stable expression of the VHL gene in A549 cells, guaranteeing high reproducibility of experimental results, which is crucial for scientific research.
Due to the importance of the VHL gene in various biological processes, the Human VHL Stable Cell Line - A549 is suitable for a wide range of research fields, including cancer research, cell biology, and drug screening.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
