Human VHL Stable Cell Line - A549

Name: Human VHL Stable Cell Line - A549
SKU: CSC-RO1122
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1122

Host Cell : A549 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1122
Description	This cell line is engineered to stably overexpress human VHL in A549 cells.
Target Gene	VHL
Gene Species	Homo sapiens (Human)
Host Cell	A549
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	VHL von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase [ Homo sapiens ]
Gene Symbol	VHL
Synonyms	VHL; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase; von Hippel Lindau syndrome , von Hippel Lindau tumor suppressor; von Hippel-Lindau disease tumor suppressor; VHL1; protein G7; elongin binding protein; RCA1; pVHL; HRCA1;
GeneID	7428
Uni ProtID	P40337
mRNA Refseq	BC058831
Chromosome Location	3p25.3
Function	enzyme binding; protein binding; transcription factor binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; ECV complex, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem;
MIM	608537

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Background

Applications

Q & A

Customer Reviews

The Human VHL Stable Cell Line, based on the A549 cell line, is designed to express the VHL (Von Hippel-Lindau) gene. VHL is a tumor suppressor gene that is crucial for regulating cell growth and preventing the formation of tumors. It functions by targeting specific proteins for degradation via the ubiquitin-proteasome system. Mutations in the VHL gene are associated with Von Hippel-Lindau disease, a hereditary condition that increases the risk of developing certain types of cancer, including renal cell carcinoma and hemangioblastoma. The A549 cell line, which is derived from a human lung carcinoma, is used for its ability to model the effects of VHL mutations in cancer. This stable cell line is instrumental for research into the mechanisms of VHL-mediated tumor suppression and for the development of targeted therapies for VHL-related cancers.

The Human VHL Stable Cell Line - A549 is instrumental for research into the VHL gene and its implications in cancer and other diseases. Its applications include:

(1) Tumor Suppression Mechanisms: VHL is a well-known tumor suppressor gene. This cell line can be used to study how VHL regulates cell growth and prevents tumor formation, providing insights into the molecular pathways that maintain cellular homeostasis.

(2) Cancer Therapy Development: Mutations in VHL are associated with Von Hippel-Lindau disease and certain cancers. Researchers can use this cell line to develop and test therapies that target VHL-dependent pathways, aiming to restore normal VHL function or compensate for its loss in cancer cells.

(3) Drug Resistance Studies: The A549 cell line can be used to investigate the mechanisms of drug resistance in cancer cells, as VHL''s role in protein degradation can influence the response to chemotherapeutic agents. Understanding these mechanisms can help in the development of strategies to overcome drug resistance.

Customer Q&As

What are the advantages of the Human VHL Stable Cell Line - A549 in studying the role of the VHL gene in tumor suppression?

A: This cell line, by stably expressing the VHL gene, provides a controllable experimental model for studying how the VHL protein functions as a tumor suppressor, helping to reveal key mechanisms of VHL in regulating cell proliferation, apoptosis, and hypoxia response.

How can the Human VHL Stable Cell Line - A549 be used to study the relationship between the VHL gene and hypoxia-inducible factors (HIF)?

A: By culturing A549 cells under hypoxic conditions, researchers can observe the impact of VHL gene expression on HIF stability and activity, thereby understanding the role of VHL in the hypoxia signaling pathway.

How can the Human VHL Stable Cell Line - A549 be applied in developing therapeutic strategies for VHL-related diseases?

A: This cell line can be used to evaluate the effects of potential therapeutic compounds on VHL function, especially in VHL-related diseases such as Von Hippel-Lindau disease, aiding in the screening of drugs that can restore normal VHL function.

How can the Human VHL Stable Cell Line - A549 be used to study the role of the VHL gene in cell cycle regulation?

A: By monitoring the relationship between VHL expression levels and cell cycle progression, researchers can explore the role of VHL in cell cycle regulation, including its effects on cyclins and cyclin-dependent kinases.

What is the value of the Human VHL Stable Cell Line - A549 in studying the connection between the VHL gene and apoptosis?

A: This cell line can be used to study the role of the VHL gene in regulating apoptosis, especially the changes in apoptotic pathways when VHL function is lost.

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Customer Reviews

Precise Gene Manipulation

The Human VHL Stable Cell Line - A549 uses precise genetic engineering techniques to stably express the VHL gene in A549 cells, providing a highly controlled environment for studying the function of VHL protein.

Germany

2023-11-30

Facilitates Tumor Research

By utilizing the Human VHL Stable Cell Line - A549, researchers can better understand the role of the VHL gene in the initiation and progression of tumors, aiding in the development of targeted cancer treatment strategies.

Canada

2022-06-29

High Reproducibility

This product ensures stable expression of the VHL gene in A549 cells, guaranteeing high reproducibility of experimental results, which is crucial for scientific research.

Canada

2022-06-29

Wide Application Prospects

Due to the importance of the VHL gene in various biological processes, the Human VHL Stable Cell Line - A549 is suitable for a wide range of research fields, including cancer research, cell biology, and drug screening.

Canada

2021-03-09

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Human VHL Stable Cell Line - A549

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What are the advantages of the Human VHL Stable Cell Line - A549 in studying the role of the VHL gene in tumor suppression?

How can the Human VHL Stable Cell Line - A549 be used to study the relationship between the VHL gene and hypoxia-inducible factors (HIF)?

How can the Human VHL Stable Cell Line - A549 be applied in developing therapeutic strategies for VHL-related diseases?

How can the Human VHL Stable Cell Line - A549 be used to study the role of the VHL gene in cell cycle regulation?

What is the value of the Human VHL Stable Cell Line - A549 in studying the connection between the VHL gene and apoptosis?

Precise Gene Manipulation

Facilitates Tumor Research

High Reproducibility

Wide Application Prospects

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