Human TLR1 mRNA

Tlr1, 2, and 6 mRNAs were transfected into A549 and MLE12 cells to test whether the mRNAs were functional. Subsequently, the cells were transfected with lipofectamine, and the expression of TLRs was detected by flow cytometry after 24 and 48 hours. Compared with samples incubated only for 24 hours after transfection, the TLR expression detected 48 hours after transfection was significantly reduced, and the number of cells was also reduced, so no further analysis was performed. Figure 1A shows a sharp increase in RFP-positive cells after transfection with RFP mRNA, which was used as a control to confirm that the lipofectamine transfection operation was successful.

Compared with untreated control cells, the level of TLR1-positive cells in A549 cells after transfection with Tlr1 mRNA was higher (Figure 1B). Compared with untransfected control cells, transfection with Tlr2 mRNA resulted in a significant increase in the level of TLR1-positive A549 cells (Figure 1C). After transfection with Tlr6 mRNA, a slight increase in TLR6-positive cells could be detected (Figure 1D).

Figure 1. TLR expression in A549 cells after transfection with Tlr mRNA. (Zeyer F S., 2017)