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Human TEK Stable Cell Line-HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO1072

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO1072
Description HEK293-TEK cell line is engineered to stably overexpress human TEK.
Target Gene TEK
Gene Species Homo sapiens (Human)
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications Drug screening and biological assays
Size >1x10^6 frozen cells/vial, 1 mL
Stability Stable in culture over a minimum of 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name TEK TEK tyrosine kinase, endothelial [ Homo sapiens ]
Gene Symbol TEK
Synonyms TIE2; VMCM; TIE-2; VMCM1; CD202B
Gene ID 7010
Uni Prot ID Q02763
m RNA Refseq NM_000459.3
Protein Refseq NP_000450.2
Chromosome Location 9p21
Function ATP binding; protein binding; protein kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity;
Pathway Angiogenesis, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem; Hemostasis, organism-specific biosystem; PI3K-Akt signaling pathway, organism-specific biosystem; PI3K-Akt signaling pathway, conserved biosystem;
MIM 600221
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