Human TEK(His) HEK293 Cell Lysate

Name: Human TEK(His) HEK293 Cell Lysate
SKU: CLOE-1251
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1251 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1251
Introduction	This cell lysate over-expresses the extracellular domain of human TEK (NP_000450.2) (Met 1-Lys 745) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	TEK
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	TEK TEK tyrosine kinase, endothelial [ Homo sapiens ]
Gene Symbol	TEK
Synonyms	TIE2; VMCM; TIE-2; VMCM1; CD202B
Gene ID	7010
Uni Prot ID	Q02763
m RNA Refseq	NM_000459.3
Protein Refseq	NP_000450.2
Chromosome Location	9p21
Function	ATP binding; protein binding; protein kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity;
Pathway	Angiogenesis, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem; Hemostasis, organism-specific biosystem; PI3K-Akt signaling pathway, organism-specific biosystem; PI3K-Akt signaling pathway, conserved biosystem;
MIM	600221

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