Human PROM1 Stable Cell Line - HEK293T

Name: Human PROM1 Stable Cell Line - HEK293T
SKU: CSC-RO0540
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0540

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0540
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Human PROM1.
Target Gene	PROM1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PROM1 prominin 1 [ Homo sapiens ]
Gene Symbol	PROM1
Synonyms	PROM1; prominin 1; macular dystrophy, retinal 2 , MCDR2, prominin (mouse) like 1 , PROML1, Stargardt disease 4 (autosomal dominant) , STGD4; prominin-1; AC133; CD133; RP41; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen; MCDR2; STGD4; CORD12; PROML1; MSTP061;
Gene ID	8842
Uni Prot ID	O43490
m RNA Refseq	BC012089
Chromosome Location	4p15
Function	actinin binding; cadherin binding; protein binding;
Pathway	Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
MIM	604365

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