Human PIK3CB_E542K Stable Cell Line - NCI-H1975

Name: Human PIK3CB_E542K Stable Cell Line - NCI-H1975
SKU: CSC-RO0454
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0454

Host Cell : NCI-H1975 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0454
Description	This cell line is derived from NCI-H1975 and is engineered to stably overexpress Human PIK3CB with E542K mutation.
Target Gene	PIK3CB
Gene Species	Homo sapiens (Human)
Host Cell	NCI-H1975
Host Cell Species	Homo sapiens (Human)
Applications	Drug screening and biological assays
Size	>1x10^6 frozen cells/vial, 1 mL
Stability	Stable in culture over a minimum of 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PIK3CB phosphoinositide-3-kinase, catalytic, beta polypeptide [ Homo sapiens ]
Gene Symbol	PIK3CB
Synonyms	PIK3CB; phosphoinositide-3-kinase, catalytic, beta polypeptide; PIK3C1; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform; PI3K-beta; PtdIns-3-kinase p110; PI3-kinase subunit beta; PI3-kinase p110 subunit beta; ptdIns-3-kinase subunit beta; ptdIns-3-kinase subunit p110-beta; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; PI3K; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237;
GeneID	5291
Uni ProtID	P42338
mRNA Refseq	BC114432
Chromosome Location	3q21-qter
Function	1-phosphatidylinositol-
Pathway	3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem;
MIM	602925

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