Human NPR3 Stable Cell Line - HEK293

Name: Human NPR3 Stable Cell Line - HEK293
SKU: CSC-SC010567-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC010567-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC010567-1
Description	This cell line is engineered to stably express human natriuretic peptide receptor 3 (NPR3) in HEK293 cells.
Target Gene	NPR3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NPR3 natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) [ Homo sapiens ]
Gene Symbol	NPR3
Synonyms	NPR3; natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C); ANPRC, C5orf23, chromosome 5 open reading frame 23 , NPRC; atrial natriuretic peptide receptor 3; FLJ14054; GUCY2B; atrial natriuretic peptide receptor type C; atrial natriuretic peptide clearance receptor; NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; C5orf23; MGC22189;
GeneID	4883
Uni ProtID	P17342
mRNA Refseq	BC131540
Chromosome Location	5p14-p13
Function	hormone binding; natriuretic peptide receptor activity; peptide hormone binding; protein homodimerization activity; receptor activity;
MIM	108962

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Background

Case Study

Applications

Q & A

Customer Reviews

The discovery of NPR3, also known as Natriuretic Peptide Receptor C, emerged from pivotal research endeavors during the mid-1980s. This receptor protein, recognized as a pivotal member of the natriuretic peptide receptor family, garnered attention due to its capacity to bind and clear circulating natriuretic peptides, thus modulating their physiological effects within cardiovascular homeostasis.

Driven by the need to elucidate the intricate molecular mechanisms underpinning NPR3 function, researchers embarked on establishing stable cell lines expressing human NPR3. Utilizing the HEK293 cell line derived from human embryonic kidney cells, which possesses favorable transfection capabilities and robust growth characteristics, served as an advantageous strategy. The introduction of human NPR3 into HEK293 cells facilitated comprehensive investigations into its intracellular signaling pathways, offering insights into its potential implications within cardiovascular pathophysiology.

Through meticulous experimentation and rigorous molecular analyses, these studies significantly advanced our comprehension of NPR3 biology and its pivotal role in cardiovascular regulation. The establishment of the Human NPR3 Stable Cell Line - HEK293 stands as a cornerstone achievement, furnishing researchers with an invaluable tool to further explore the physiological and pathological ramifications of NPR3 signaling pathways.

Mice transmit itch via separate neural pathways: one histamine-dependent, the other histamine-independent. These pathways express gastrin-releasing peptide (GRP) and neuromedin B (NMB), respectively, to the spinal cord. Researchers demonstrate that NPR3, encoding the BNP receptor NPRC, plays a crucial role in mediating histaminergic itch transmission. NPR3 expression overlaps with NMBR in the dorsal horn, implicated in histaminergic itch via Gq-coupled signaling. NPRC is required for histamine-induced itch but not non-histaminergic itch. Importantly, BNP enhances scratching behaviors mediated by NMB, not GRP, indicating NPRC-NMBR cross-signaling. These findings unveil a novel mechanism whereby BNP facilitates NMB-encoded itch through NPRC-NMBR interaction, revealing distinct neuropeptide actions in itch modulation.

NPR3 role in the molecular profile of the spinal cord, particularly its co-expression with Nmbr and Vglut2 in laminae I-II, is demonstrated by researchers. Figure 1. NPR3's role in the molecular profile of the spinal cord, particularly its co-expression with Nmbr and Vglut2 in laminae I-II, is demonstrated by researchers. (Meng QT, et al., 2021)

The utilization of Creative Biogene's Human NPR3 Stable Cell Line - HEK293 may enhance experimental approaches similar to those described, offering a controlled in vitro system for investigating NPR3-related molecular interactions and signaling pathways.

1. Signal Transduction Studies: Investigate intracellular signaling pathways activated by NPR3 through assays like Western blotting and immunofluorescence.

2. Disease Modeling: Establish disease models by transfecting cells with specific gene mutations associated with NPR3 dysregulation.

3. Pharmacological Studies: Evaluate the binding affinity and pharmacokinetics of novel NPR3-targeting compounds using radioligand binding assays.

4. Functional Analysis: Assess the impact of NPR3 modulation on cellular functions such as proliferation, apoptosis, and ion transport.

5. Transgenic Animal Validation: Validate findings from cell-based assays by studying NPR3 function in transgenic animal models, correlating with cellular responses observed in vitro.

Customer Q&As

What factors influenced the selection of HEK293 cells for establishing the stable NPR3 cell line?

A: HEK293 cells were likely chosen for their robust expression machinery, allowing for efficient production of membrane-bound proteins like NPR3, essential for studying its role in cardiovascular physiology.

How was the stability of NPR3 expression verified and maintained in this HEK293 stable cell line?

A: Stability was likely confirmed through methods such as immunoblotting, flow cytometry, or functional assays assessing NPR3-mediated cGMP signaling, with continuous selection pressure applied.

Can you elaborate on the characterization of NPR3 expression in the HEK293 stable cell line, including its subcellular localization and ligand-binding properties?

A: Characterization may involve analysis of NPR3 localization, ligand-binding kinetics, downstream signaling pathways, and functional implications in blood pressure regulation and cardiovascular homeostasis.

What quality control measures were employed during the generation of this stable cell line?

A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.

How does the expression pattern and functional properties of NPR3 in this stable cell line correspond to its physiological roles and relevance in cardiovascular diseases and therapeutic interventions?

A: Comparative analysis with primary cardiomyocytes or animal models helps validate the relevance of NPR3 expression in blood pressure regulation, heart failure, and potential pharmacological targeting for cardiovascular therapies.

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Customer Reviews

Consistent GFRAL expression

This Human NPR3 Stable Cell Line is a gem! Its stable expression in HEK293 cells has revolutionized my research on natriuretic peptide signaling.

United States

2023-03-17

Gem NPR3 expression

Using this cell line feels like having a secret weapon! The stable NPR3 expression has made studying cardiovascular function pathways a breeze.

Germany

2020-09-23

Easy cardiovascular function study

Can't believe how much easier my experiments have become with this cell line! Its reliable expression in HEK293 cells has elevated the quality of my research on hypertension.

Germany

2020-08-29

Secret weapon NPR3 expression

So impressed with this Human NPR3 Stable Cell Line! Its consistent expression has given me peace of mind and boosted the credibility of my findings.

Germany

2023-03-02

Reliable NPR3 expression

Huge shoutout to this cell line for simplifying my work! With stable NPR3 expression, I can delve deeper into cardiovascular function without any worries.

Germany

2023-04-23

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Human NPR3 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What factors influenced the selection of HEK293 cells for establishing the stable NPR3 cell line?

How was the stability of NPR3 expression verified and maintained in this HEK293 stable cell line?

Can you elaborate on the characterization of NPR3 expression in the HEK293 stable cell line, including its subcellular localization and ligand-binding properties?

What quality control measures were employed during the generation of this stable cell line?

How does the expression pattern and functional properties of NPR3 in this stable cell line correspond to its physiological roles and relevance in cardiovascular diseases and therapeutic interventions?

Consistent GFRAL expression

Gem NPR3 expression

Easy cardiovascular function study

Secret weapon NPR3 expression

Reliable NPR3 expression

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