Transfected Stable Cell Lines
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Cat. No. : LV29059L
Storage : -80℃ Shipping : Frozen on dry ice
Titer: Size:
| Cat. No. | LV29059L |
| Description | Lentiviral particles of thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 3. |
| Product Type | Premade Lentivirus Particles |
| Gene | TXNDC5 |
| Species | Human |
| Insert Size | 975 bp |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
| Gene Name | TXNDC5 thioredoxin domain containing 5 (endoplasmic reticulum) [ Homo sapiens ] |
| Gene Symbol | TXNDC5 |
| Synonyms | TXNDC5; thioredoxin domain containing 5 (endoplasmic reticulum); thioredoxin domain containing 5; thioredoxin domain-containing protein 5; EndoPDI; ERp46; FLJ21353; FLJ90810; Hcc 2; MGC3178; PDIA15; protein disulfide isomerase family A; member 15; ER protein 46; thioredoxin related protein; thioredoxin-like protein p46; endoplasmic reticulum protein ERp46; endothelial protein disulphide isomerase; endoplasmic reticulum resident protein 46; protein disulfide isomerase family A, member 15; ERP46; HCC-2; STRF8; UNQ364; ENDOPDI; FLJ21789; |
| Gene ID | 81567 |
| Uni Prot ID | Q8NBS9 |
| m RNA Refseq | BC052310 |
| Chromosome Location | 6p24.3 |
| Function | electron carrier activity; isomerase activity; protein disulfide oxidoreductase activity; |
| Pathway | Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Lysosome Vesicle Biogenesis, organism-specific biosystem; Membrane Trafficking, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem; |
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