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human TOP2B (NM_001068) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV28425L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV28425L
Description Lentiviral particles of topoisomerase (DNA) II beta 180kDa (TOP2B).
Product Type Premade Lentivirus Particles
Gene TOP2B
Species Human
Insert Size 4866 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name TOP2B topoisomerase (DNA) II beta 180kDa [ Homo sapiens ]
Gene Symbol TOP2B
Synonyms TOPIIB; top2beta
Gene Description topoisomerase (DNA) II beta 180kDa
Gene ID 7155
Uni Prot ID Q02880
m RNA Refseq NM_001068.2
Protein Refseq NP_001059.2
Chromosome Location 3p24
Function ATP binding; DNA topoisomerase (ATP-hydrolyzing) activity; chromatin binding; enzyme binding; histone deacetylase binding; protein C-terminus binding; protein heterodimerization activity; protein kinase C binding;
MIM 126431
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