Transfected Stable Cell Lines
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Cat. No. : LV27462L
Storage : -80℃ Shipping : Frozen on dry ice
Titer: Size:
| Cat. No. | LV27462L |
| Description | Lentiviral particles of transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) (TCEB3). |
| Product Type | Premade Lentivirus Particles |
| Gene | TCEB3 |
| Species | Human |
| Insert Size | 2397 bp |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
| Gene Name | TCEB3 transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A) [ Homo sapiens ] |
| Gene Symbol | TCEB3 |
| Synonyms | TCEB3; transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A); transcription elongation factor B (SIII), polypeptide 3 (110kD, elongin A); transcription elongation factor B polypeptide 3; SIII; TCEB3A; elongin-A; elongin 110 kDa subunit; transcription elongation factor B alpha subunit; RNA polymerase II transcription factor SIII subunit A1; EloA; SIII p110; FLJ38760; FLJ42849; |
| Gene ID | 6924 |
| Uni Prot ID | Q14241 |
| m RNA Refseq | BC002883 |
| Chromosome Location | 1p36.1 |
| Pathway | Disease, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem; Formation of RNA Pol II elongation complex, organism-specific biosystem; Gene Expression, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem; |
| MIM | 600786 |
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