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human SPEN (NM_015001) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV26475L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV26475L
Description Lentiviral particles of spen homolog, transcriptional regulator (Drosophila) (SPEN).
Product Type Premade Lentivirus Particles
Gene SPEN
Species Human
Insert Size 10995 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name SPEN spen homolog, transcriptional regulator (Drosophila) [ Homo sapiens ]
Gene Symbol SPEN
Synonyms MINT; SHARP; RBM15C; HIAA0929; RP1-134O19.1
Gene Description spen homolog, transcriptional regulator (Drosophila)
Gene ID 23013
Uni Prot ID Q96T58
m RNA Refseq NM_015001.2
Protein Refseq NP_055816.2
Chromosome Location 1p36
Function DNA binding; RNA binding; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription; nucleotide binding; protein binding;
Pathway Delta-Notch Signaling Pathway, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem;
MIM 613484
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